TLN1 antibody (C-Term)

Catalog No. ABIN2855327

The Rabbit Polyclonal anti-TLN1 antibody has been validated for WB and IHC (p). It is suitable to detect TLN1 in samples from Human.

Quick Overview for TLN1 antibody (C-Term) (ABIN2855327)

Target: TLN1 (Talin 1 (TLN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TLN1 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to Talin-1 (talin 1)
Talin-1 antibody [C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Talin-1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.09 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for TLN1

Target: TLN1 (Talin 1 (TLN1))

Alternative Name: talin 1

Background: This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.

Cellular Localization: Cell projection , ruffle membrane, Peripheral membrane protein, Cytoplasmic side (By similarity) , Cytoplasm , cytoskeleton (By similarity)

Molecular Weight: 270 kDa

Gene ID: 7094

UniProt: Q9Y490

Pathways: Cell-Cell Junction Organization, ER-Nucleus Signaling, Maintenance of Protein Location