ERP29 antibody

Catalog No. ABIN2855330

The Rabbit Polyclonal anti-ERP29 antibody has been validated for WB, IHC (p), ICC and IF. It is suitable to detect ERP29 in samples from Human. There is 1 publication available.

Quick Overview for ERP29 antibody (ABIN2855330)

Target: ERP29 (Endoplasmic Reticulum Protein 29 (ERP29))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERP29 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-ERP29 Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to ERP29 (endoplasmic reticulum protein 29)
ERP29 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ERP29. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , 293T , A431 , H1299

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-ERP29 antibody (ABIN2855330)

Guo, Ma, Liu, Lei, Tang, Huang, Huang, Li, Wang, Liu, Tang, Jing, Deng: "ERp29 counteracts the suppression of malignancy mediated by endoplasmic reticulum stress and promotes the metastasis of colorectal cancer." in: Oncology reports, Vol. 41, Issue 3, pp. 1603-1615, (2019) (PubMed).

Target Details for ERP29

Target: ERP29 (Endoplasmic Reticulum Protein 29 (ERP29))

Alternative Name: endoplasmic reticulum protein 29

Background: This gene encodes a reticuloplasmin, a protein which resides in the lumen of the endoplasmic reticulum (ER). The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms.

Cellular Localization: Endoplasmic reticulum lumen , Melanosome

Molecular Weight: 29 kDa

Gene ID: 10961

UniProt: P30040