MYB antibody (Internal Region)

Catalog No. ABIN2855353

This Rabbit Polyclonal antibody specifically detects MYB in WB. It exhibits reactivity toward Human.

Quick Overview for MYB antibody (Internal Region) (ABIN2855353)

Target: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYB antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MYB Antibody

Binding Specificity: Internal Region

Cross-Reactivity: Mouse (Murine), Cow (Bovine)

Cross-Reactivity (Details): Mouse (83 %), Bovine (91 %)

Characteristics: Rabbit Polyclonal antibody to c-Myb (v-myb myeloblastosis viral oncogene homolog (avian))
c-Myb antibody [N2C1], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human c-Myb. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceWestern blot1:500-1:3000*

Comment:

Positive Control: A549 , HeLa , HepG2 , HCT116

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for MYB

Target: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Alternative Name: C-Myb

Background: This gene encodes a transcription factor that is a member of the MYB family of transcription factor genes. The protein contains three domains, an N-terminal DNA-binding domain, a central transcriptional activation domain and a C-terminal domain involved in transcriptional repression. This protein plays an essential role in the regulation of hematopoiesis and may play a role in tumorigenesis. Alternative splicing results in multiple transcript variants.

Cellular Localization: Nucleus

Molecular Weight: 72 kDa

Gene ID: 4602

Pathways: Chromatin Binding, Positive Regulation of Immune Effector Process