CAPN1 antibody

Catalog No. ABIN2855363

The Rabbit Polyclonal anti-CAPN1 antibody has been validated for WB, ICC, IP, IHC (p) and IF. It is suitable to detect CAPN1 in samples from Human.

Quick Overview for CAPN1 antibody (ABIN2855363)

Target: CAPN1 (Calpain 1 (CAPN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAPN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-CAPN1 Antibody

Cross-Reactivity: Human, Mouse, Squirrel

Characteristics: Rabbit Polyclonal antibody to Calpain 1 (calpain 1, (mu/I) large subunit)
Calpain 1 antibody [N3C2], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Calpain 1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.97 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for CAPN1

Target: CAPN1 (Calpain 1 (CAPN1))

Alternative Name: calpain 1

Background: The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1.

Molecular Weight: 82 kDa

Gene ID: 823

UniProt: P07384