CAPN1 antibody (Middle Region)
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- Target See all CAPN1 Antibodies
- CAPN1 (Calpain 1 (CAPN1))
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Binding Specificity
- Middle Region
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CAPN1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Purification
- Antigen affinity
- Immunogen
- An amino acid sequence from the middle region of human CAPNL1 (EWNNVDPYERDQLRV) was used as the immunogen for this Calpain 1 antibody.
- Isotype
- IgG
- Top Product
- Discover our top product CAPN1 Primary Antibody
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- Application Notes
- The stated application concentrations are suggested starting amounts. Titration of the Calpain 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL
- Restrictions
- For Research Use only
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- Buffer
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
- Storage
- -20 °C
- Storage Comment
- After reconstitution, the Calpain 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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- Target
- CAPN1 (Calpain 1 (CAPN1))
- Alternative Name
- Calpain 1 (CAPN1 Products)
- Synonyms
- CAPN1 antibody, Capa-1 antibody, Capa1 antibody, mu-calpin antibody, Capn1 antibody, fj67g01 antibody, wu:fj67g01 antibody, calpain 1 antibody, zgc:55262 antibody, CAPN1 antibody, Capn1 antibody, zgc:55262 antibody
- Background
- Calpain-1 catalytic subunit is a protein that in humans is encoded by the CAPN1 gene. Calpain is an intracellular protease that requires calcium for its catalytic activity. Two isozymes, CAPN1 (mu-calpain) and CAPN2 (m-calpain), with different calcium requirements, have been identified. Both are heterodimers composed of L (large, catalytic, 82 kD) and S (small, regulatory, 30 kD) subunits. The isozymes share an identical S subunit, with the differences arising from the L subunits. By quantitative RT-PCR, Ueyama et al.(1998) found that expression of Calpain1/2 mRNA was significantly increased in muscle biopsy samples derived from 5 men with progressive muscular dystrophy (e.g., DMD, 310200) and 2 men and 3 women with amyotrophic lateral sclerosis(ALS, 105400) compared with controls. Using cDNA clones as probes, Ohno et al.(1989, 1990) assign CAPNL1 to chromosome 11.
- UniProt
- P07384
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