p63 antibody

Catalog No. ABIN2855394

This anti-p63 antibody is a Rabbit Polyclonal antibody detecting p63 in WB, ICC, IF, IP, IHC (p), IHC (fro) and PLA. Suitable for Human. This Primary Antibody has been cited in 8+ publications.

Quick Overview for p63 antibody (ABIN2855394)

Target: p63 (TP63) (Tumor Protein P63 (TP63))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p63 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Proximity Ligation Assay (PLA)

Grade: KO Validated

Product Details anti-p63 Antibody

Cross-Reactivity: Dog, Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to p63 (tumor protein p63)
p63 antibody [N2C1], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human p63. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: mouse brain , rat brain , A431

Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.58 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-p63 antibody (ABIN2855394)

Kim, Dollé, Vermeij, Gyenis, Vogel, Hoeijmakers, Wiley, Davalos, Hasty, Desprez, Campisi: "Deficiency in the DNA repair protein ERCC1 triggers a link between senescence and apoptosis in human fibroblasts and mouse skin." in: Aging cell, Vol. 19, Issue 3, pp. e13072, (2020) (PubMed).

Li, Wang, Torkelson, Shankar, Pattison, Zhen, Fang, Duren, Xin, Gaddam, Melo, Piekos, Li, Liaw, Chen, Li, Wernig, Wong, Chang, Oro: "TFAP2C- and p63-Dependent Networks Sequentially Rearrange Chromatin Landscapes to Drive Human Epidermal Lineage Commitment." in: Cell stem cell, Vol. 24, Issue 2, pp. 271-284.e8, (2020) (PubMed).

Jiang, Zhu, He, Gao, Tian, Ma, Wu, Das, Severinov, Hitzeroth, Debata, Liu, Zou, Shi, Xu, Wang, Bao, Ka-Kit, You, Cui, Hu: "Cytological Immunostaining of HMGA2, LRP1B, and TP63 as Potential Biomarkers for Triaging Human Papillomavirus-Positive Women." in: Translational oncology, Vol. 12, Issue 7, pp. 959-967, (2019) (PubMed).

Montoro, Haber, Biton, Vinarsky, Lin, Birket, Yuan, Chen, Leung, Villoria, Rogel, Burgin, Tsankov, Waghray, Slyper, Waldman, Nguyen, Dionne, Rozenblatt-Rosen, Tata, Mou, Shivaraju, Bihler, Mense et al.: "A revised airway epithelial hierarchy includes CFTR-expressing ionocytes. ..." in: Nature, Vol. 560, Issue 7718, pp. 319-324, (2019) (PubMed).

Xiong, Ren, Chen, Liu, Huang, Zhang, Odera, Chen, Kist, Peters, Garman, Sun, Chen: "PAX9 regulates squamous cell differentiation and carcinogenesis in the oro-oesophageal epithelium." in: The Journal of pathology, Vol. 244, Issue 2, pp. 164-175, (2019) (PubMed).

OBrien, Dorsey, Hannan, Hultgren: "Host restriction of Escherichia coli recurrent urinary tract infection occurs in a bacterial strain-specific manner." in: PLoS pathogens, Vol. 14, Issue 12, pp. e1007457, (2019) (PubMed).

Reneker, Wang, Irlmeier, Huang: "Fibroblast Growth Factor Receptor 2 (FGFR2) Is Required for Meibomian Gland Homeostasis in the Adult Mouse." in: Investigative ophthalmology & visual science, Vol. 58, Issue 5, pp. 2638-2646, (2017) (PubMed).

OBrien, Hannan, Yu, Livny, Roberson, Schwartz, Souza, Mendelsohn, Colonna, Lewis, Hultgren: "A mucosal imprint left by prior Escherichia coli bladder infection sensitizes to recurrent disease." in: Nature microbiology, Vol. 2, pp. 16196, (2016) (PubMed).

Target Details for p63

Target: p63 (TP63) (Tumor Protein P63 (TP63))

Alternative Name: tumor protein p63

Background: This gene encodes a member of the p53 family of transcription factors. An animal model, p63 -/- mice, has been useful in defining the role this protein plays in the development and maintenance of stratified epithelial tissues. p63 -/- mice have several developmental defects which include the lack of limbs and other tissues, such as teeth and mammary glands, which develop as a result of interactions between mesenchyme and epithelium. Mutations in this gene are associated with ectodermal dysplasia, and cleft lip/palate syndrome 3 (EEC3), split-hand/foot malformation 4 (SHFM4), ankyloblepharon-ectodermal defects-cleft lip/palate, ADULT syndrome (acro-dermato-ungual-lacrimal-tooth), limb-mammary syndrome, Rap-Hodgkin syndrome (RHS), and orofacial cleft 8. Both alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different proteins. Many transcripts encoding different proteins have been reported but the biological validity and the full-length nature of these variants have not been determined.

Cellular Localization: Nucleus

Molecular Weight: 77 kDa

Gene ID: 8626

UniProt: Q9H3D4