PTPN12 antibody (N-Term)

Catalog No. ABIN2855422

This Rabbit Polyclonal antibody specifically detects PTPN12 in WB, IHC (p), IF and ICC. It exhibits reactivity toward Human.

Quick Overview for PTPN12 antibody (N-Term) (ABIN2855422)

Target: PTPN12 (Protein Tyrosine Phosphatase G1 (PTPN12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTPN12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PTPN12 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human, Rat

Characteristics: Rabbit Polyclonal antibody to PTPN12 (protein tyrosine phosphatase, non-receptor type 12)
PTPN12 antibody [N1N2], N-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human PTPN12. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.93 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PTPN12

Target: PTPN12 (Protein Tyrosine Phosphatase G1 (PTPN12))

Alternative Name: protein tyrosine phosphatase non-receptor type 12

Background: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains a C-terminal PEST motif, which serves as a protein-protein interaction domain, and may regulate protein intracellular half-life. This PTP was found to bind and dephosphorylate the product of the oncogene c-ABL and thus may play a role in oncogenesis. This PTP was also shown to interact with, and dephosphorylate, various products related to cytoskeletal structure and cell adhesion, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin. This suggests it has a regulatory role in controlling cell shape and mobility. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Cellular Localization: Cytoplasm

Molecular Weight: 88 kDa

Gene ID: 5782

UniProt: Q05209