GRP78 antibody (Internal Region)

Catalog No. ABIN2855429

The Rabbit Polyclonal anti-GRP78 antibody has been validated for WB, IF, ICC, IP and IHC (p). It is suitable to detect GRP78 in samples from Human, Rat and Mouse. There are 2+ publications available.

Quick Overview for GRP78 antibody (Internal Region) (ABIN2855429)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRP78 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GRP78 Antibody

Binding Specificity: Internal Region

Cross-Reactivity: Chicken, Cow (Bovine), Rhesus Monkey

Cross-Reactivity (Details): Mouse (100 %), Rat (100 %), Chicken (100 %), Rhesus Monkey (100 %), Bovine (100 %)

Characteristics: Rabbit polyclonal antibody to Grp78 (heat shock 70 kDa protein 5 (glucose-regulated protein, 78 kDa))
Grp78 antibody [N2C1], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Grp78. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:1000-1:10000*

Comment:

Positive Control: 293T , A431 , HeLa , HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.72 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-GRP78 antibody (ABIN2855429)

Cai, Han, Hu, Zhao, He, Chen, Sun, Yuan, Li, Yang, Kong, Kong: "Metformin attenuates the D‑galactose‑induced aging process via the UPR through the AMPK/ERK1/2 signaling pathways." in: International journal of molecular medicine, Vol. 45, Issue 3, pp. 715-730, (2020) (PubMed).

Chen, Zhao, Cai, Sun, Hu, Huang, Kong, Kong: "The role of sodium hydrosulfide in attenuating the aging process via PI3K/AKT and CaMKKβ/AMPK pathways." in: Redox biology, Vol. 12, pp. 987-1003, (2017) (PubMed).

Target Details for GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Alternative Name: Grp78

Background: When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]

Cellular Localization: Endoplasmic reticulum lumen , Melanosome

Molecular Weight: 72 kDa

Gene ID: 3309

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling