Transferrin Receptor antibody

Catalog No. ABIN2855434

Product Details anti-Transferrin Receptor Antibody

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Transferrin Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to CD71 (transferrin receptor (p90, CD71))
CD71 antibody [N2C1], Internal

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human CD71. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Jurkat , Raji , NCI-H929 , K562 , HL-60 , NIH3T3 , HeLa

Validation: Comparison, KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.47 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-Transferrin Receptor antibody (ABIN2855434)

Conde-Rubio, Mylonas, Widmann: "The proteolytic landscape of cells exposed to non-lethal stresses is shaped by executioner caspases." in: Cell death discovery, Vol. 7, Issue 1, pp. 164, (2021) (PubMed).

Singh, Leu, Barnoud, Vonteddu, Gnanapradeepan, Lin, Liu, Barton, Kossenkov, George, Murphy, Dotiwala: "African-centric TP53 variant increases iron accumulation and bacterial pathogenesis but improves response to malaria toxin." in: Nature communications, Vol. 11, Issue 1, pp. 473, (2020) (PubMed).

Target Details for Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: transferrin receptor (TFRC Products)

Background: Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.

Cellular Localization: Cell membrane, Single-pass type II membrane protein , Melanosome , Transferrin receptor protein 1 , serum form: Secreted

Molecular Weight: 85 kDa

Gene ID: 7037

UniProt: P02786

Pathways: Transition Metal Ion Homeostasis