P4HA2 antibody

Catalog No. ABIN2855459

The Rabbit Polyclonal anti-P4HA2 antibody has been validated for WB. It is suitable to detect P4HA2 in samples from Human.

Quick Overview for P4HA2 antibody (ABIN2855459)

Target: P4HA2 (Prolyl 4-Hydroxylase, alpha Polypeptide II (P4HA2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P4HA2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-P4HA2 Antibody

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to P4HA2 (procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II)
P4HA2 antibody [N1C3]

Purification: Affinity purified by Protein A.

Immunogen: Recombinant protein encompassing a sequence within the center region of human P4HA2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: H1299

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.82 mg/mL

Buffer: 1XPBS ( pH 7), 40 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for P4HA2

Target: P4HA2 (Prolyl 4-Hydroxylase, alpha Polypeptide II (P4HA2))

Alternative Name: prolyl 4-hydroxylase subunit alpha 2

Background: This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

Cellular Localization: Endoplasmic reticulum lumen

Molecular Weight: 61 kDa

Gene ID: 8974

UniProt: O15460