PPAT antibody

Catalog No. ABIN2855469

This anti-PPAT antibody is a Rabbit Polyclonal antibody detecting PPAT in WB, IF and ICC. Suitable for Human.

Quick Overview for PPAT antibody (ABIN2855469)

Target: PPAT (Phosphoribosyl Pyrophosphate Amidotransferase (PPAT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPAT antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PPAT Antibody

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to PPAT (amidophosphoribosyltransferase)
PPAT antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PPAT. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HepG2 , HCT116

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PPAT

Target: PPAT (Phosphoribosyl Pyrophosphate Amidotransferase (PPAT))

Alternative Name: phosphoribosyl pyrophosphate amidotransferase

Background: The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. This protein is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. This gene and PAICS/AIRC, a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway, are located in close proximity on chromosome 4.

Molecular Weight: 57 kDa

Gene ID: 5471

UniProt: Q06203