ATP Synthase B1 (Center) antibody

Catalog No. ABIN2855527

This Rabbit Polyclonal antibody specifically detects in ICC, IF, IHC (p) and WB. It exhibits reactivity toward Human and Mouse.

Quick Overview for ATP Synthase B1 (Center) antibody (ABIN2855527)

Target: ATP Synthase B1

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Application: Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Product Details

Binding Specificity: Center

Characteristics: Rabbit Polyclonal antibody to ATP synthase B1 (ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1)
ATP synthase B1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ATP synthase B1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: Molt-4 , mouse brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.45 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details

Target: ATP Synthase B1

Background: This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel.

Cellular Localization: Mitochondrion , Mitochondrion inner membrane

Molecular Weight: 29 kDa

Gene ID: 515