BHMT antibody

Catalog No. ABIN2855528

The Rabbit Polyclonal anti-BHMT antibody has been validated for WB, IF, ICC and IHC (p). It is suitable to detect BHMT in samples from Human.

Quick Overview for BHMT antibody (ABIN2855528)

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BHMT antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BHMT Antibody

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to BHMT (betaine-homocysteine methyltransferase)
BHMT antibody [N3C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human BHMT. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HepG2 , mouse liver

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for BHMT

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Alternative Name: betaine--homocysteine S-methyltransferase

Background: This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.

Molecular Weight: 45 kDa

Gene ID: 635

UniProt: Q93088

Pathways: Methionine Biosynthetic Process