SERPINH1 antibody (Internal Region)

Catalog No. ABIN2855536

This Rabbit Polyclonal antibody specifically detects SERPINH1 in WB and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for SERPINH1 antibody (Internal Region) (ABIN2855536)

Target: SERPINH1 (Serpin Family H Member 1 (SERPINH1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SERPINH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SERPINH1 Antibody

Binding Specificity: Internal Region

Cross-Reactivity: Chimpanzee, Rhesus Monkey

Cross-Reactivity (Details): Rhesus Monkey (100 %), Chimpanzee (100 %)

Characteristics: Rabbit Polyclonal antibody to HSP47 (serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1))
HSP47 antibody [N2C2], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human HSP47. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: NIH-3T3 , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.37 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for SERPINH1

Target: SERPINH1 (Serpin Family H Member 1 (SERPINH1))

Alternative Name: HSP47

Background: This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen, thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis.

Cellular Localization: Endoplasmic reticulum lumen

Molecular Weight: 46 kDa

Gene ID: 871