STIP1 antibody

Catalog No. ABIN2855539

This Rabbit Polyclonal antibody specifically detects STIP1 in WB, IF, IHC (p) and ICC. It exhibits reactivity toward Human.

Quick Overview for STIP1 antibody (ABIN2855539)

Target: STIP1 (Stress-Induced-phosphoprotein 1 (STIP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STIP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-STIP1 Antibody

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to STIP1 (stress-induced-phosphoprotein 1)
STIP1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human STIP1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Jurkat , Raji , K562 , THP-1

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.89 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for STIP1

Target: STIP1 (Stress-Induced-phosphoprotein 1 (STIP1))

Alternative Name: stress induced phosphoprotein 1

Background: STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A, MIM 140550) and HSP90 (see HSP90AA1, MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).[supplied by OMIM]

Cellular Localization: Cytoplasm (By similarity) , Nucleus

Molecular Weight: 63 kDa

Gene ID: 10963

UniProt: P31948