NRF1 antibody

Catalog No. ABIN2855569

This Rabbit Polyclonal antibody specifically detects NRF1 in WB, IHC (p), IF, ICC and IP. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for NRF1 antibody (ABIN2855569)

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NRF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-NRF1 Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to NRF1 (nuclear respiratory factor 1)
NRF1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human NRF1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T nuclear , A431 nuclear , HeLa nuclear , HepG2 nuclear

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.32 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-NRF1 antibody (ABIN2855569)

Mesquita, Lamb, Parry, Moore, Smith, Vann, Osburn, Fox, Ruple, Huggins, Fruge, Young, Kavazis, Roberts: "Acute and chronic effects of resistance training on skeletal muscle markers of mitochondrial remodeling in older adults." in: Physiological reports, Vol. 8, Issue 15, pp. e14526, (2020) (PubMed).

Ma, Cheng, Mu, Geng, Zhao, Luo, Ma, Chang, Liu, Gao, Nie, Xie, Han, Chen, Ma, Zhu, Chen: "The SIAH2-NRF1 axis spatially regulates tumor microenvironment remodeling for tumor progression." in: Nature communications, Vol. 10, Issue 1, pp. 1034, (2019) (PubMed).

Target Details for NRF1

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Alternative Name: nuclear respiratory factor 1

Background: This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1.

Cellular Localization: Nucleus

Molecular Weight: 54 kDa

Gene ID: 4899

UniProt: Q16656

Pathways: Regulation of Lipid Metabolism by PPARalpha