NOV antibody (Center)

Catalog No. ABIN2855618

The Rabbit Polyclonal anti-NOV antibody has been validated for WB, IHC (p), ICC and IF. It is suitable to detect NOV in samples from Human and Mouse.

Quick Overview for NOV antibody (Center) (ABIN2855618)

Target: NOV (Nephroblastoma Overexpressed (NOV))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOV antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-NOV Antibody

Binding Specificity: Center

Cross-Reactivity: Rhesus Monkey

Cross-Reactivity (Details): Rhesus Monkey (100 %)

Characteristics: Rabbit Polyclonal antibody to CCN3 (nephroblastoma overexpressed gene)
CCN3 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human CCN3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:10000*

Comment:

Positive Control: 293T , mouse brain , human CCN3-transfected 293T cells

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NOV

Target: NOV (Nephroblastoma Overexpressed (NOV))

Alternative Name: CCN3

Background: The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development.

Cellular Localization: Secreted

Molecular Weight: 39 kDa

Gene ID: 4856

Pathways: Smooth Muscle Cell Migration, Growth Factor Binding