DNAJB12 antibody (Center)

Catalog No. ABIN2855671

This Rabbit Polyclonal antibody specifically detects DNAJB12 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for DNAJB12 antibody (Center) (ABIN2855671)

Target: DNAJB12 (DnaJ (Hsp40) Homolog, Subfamily B, Member 12 (DNAJB12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNAJB12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DNAJB12 Antibody

Binding Specificity: Center

Cross-Reactivity: Mouse (Murine), Chicken, Rat (Rattus), Xenopus laevis, Cow (Bovine), Xenopus tropicalis

Cross-Reactivity (Details): Mouse (96 %), Chicken (85 %), Rat (96 %), Xenopus laevis (80 %), Bovine (94 %), Xenopus tropicalis (82 %)

Characteristics: Rabbit Polyclonal antibody to DNAJB12 (DnaJ (Hsp40) homolog, subfamily B, member 12)
DNAJB12 antibody [N2C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human DNAJB12. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: A549 , H1299 , HCT116 , MCF-7

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.89 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for DNAJB12

Target: DNAJB12 (DnaJ (Hsp40) Homolog, Subfamily B, Member 12 (DNAJB12))

Alternative Name: DNAJB12

Background: DNAJB12 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus, a glycine/phenylalanine (G/F)-rich region, and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by OMIM]

Cellular Localization: Membrane

Molecular Weight: 42 kDa

Gene ID: 54788