DFFA antibody

Catalog No. ABIN2855675

The Rabbit Polyclonal anti-DFFA antibody has been validated for WB. It is suitable to detect DFFA in samples from Human.

Quick Overview for DFFA antibody (ABIN2855675)

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DFFA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DFFA Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to ICAD (DNA fragmentation factor, 45 kDa, alpha polypeptide)
ICAD antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ICAD. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , Jurkat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for DFFA

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Alternative Name: DNA fragmentation factor subunit alpha

Background: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Cellular Localization: Cytoplasm

Molecular Weight: 37 kDa

Gene ID: 1676

UniProt: O00273

Pathways: Apoptosis, Caspase Cascade in Apoptosis