ENO3 antibody

Catalog No. ABIN2855707

This Rabbit Polyclonal antibody specifically detects ENO3 in WB, ICC and IF. It exhibits reactivity toward Human.

Quick Overview for ENO3 antibody (ABIN2855707)

Target: ENO3 (Enolase 3 (Beta, Muscle) (ENO3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENO3 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-ENO3 Antibody

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to ENO3 (enolase 3 (beta, muscle))
ENO3 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ENO3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ENO3

Target: ENO3 (Enolase 3 (Beta, Muscle) (ENO3))

Alternative Name: enolase 3

Background: This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for this gene that differ only in their 5' UTR.

Cellular Localization: Cytoplasm

Molecular Weight: 47 kDa

Gene ID: 2027

UniProt: P13929