PSMA5 antibody (Center)

Catalog No. ABIN2855714

The Rabbit Polyclonal anti-PSMA5 antibody has been validated for WB, IF, IHC (p) and ICC. It is suitable to detect PSMA5 in samples from Human and Mouse.

Quick Overview for PSMA5 antibody (Center) (ABIN2855714)

Target: PSMA5 (Proteasome Subunit alpha 5 (PSMA5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMA5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-PSMA5 Antibody

Binding Specificity: Center

Cross-Reactivity: Pig (Porcine), Rhesus Monkey, Cow (Bovine)

Cross-Reactivity (Details): Pig (100 %), Rhesus Monkey (100 %), Bovine (100 %)

Characteristics: Rabbit Polyclonal antibody to PSMA5 (proteasome (prosome, macropain) subunit, alpha type, 5)
PSMA5 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PSMA5. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:10000*

Comment:

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.96 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PSMA5

Target: PSMA5 (Proteasome Subunit alpha 5 (PSMA5))

Alternative Name: PSMA5

Background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit.

Cellular Localization: Cytoplasm , Nucleus

Molecular Weight: 26 kDa

Gene ID: 5686

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA