IP6K1 antibody

Catalog No. ABIN2855731

This Rabbit Polyclonal antibody specifically detects IP6K1 in WB, IP, ICC, IHC (p) and IF. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for IP6K1 antibody (ABIN2855731)

Target: IP6K1 (Inositol Hexakisphosphate Kinase 1 (IP6K1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IP6K1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Grade: KO Validated

Product Details anti-IP6K1 Antibody

Specificity: Knockdown/Knockout validation was supported by references (PMID:27701146)

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to IP6K1 (inositol hexakisphosphate kinase 1)
IP6K1 antibody

Purification: Affinity purified by Protein A.

Immunogen: Recombinant protein encompassing a sequence within the center region of human IP6K1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Jurkat , Raji , NCI-H929

Validation: KO/KD, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.22 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-IP6K1 antibody (ABIN2855731)

Zhu, Ghoshal, Tyagi, Chakraborty: "Global IP6K1 deletion enhances temperature modulated energy expenditure which reduces carbohydrate and fat induced weight gain." in: Molecular metabolism, Vol. 6, Issue 1, pp. 73-85, (2018) (PubMed).

Target Details for IP6K1

Target: IP6K1 (Inositol Hexakisphosphate Kinase 1 (IP6K1))

Alternative Name: inositol hexakisphosphate kinase 1

Background: This gene encodes a protein that belongs to the inositol phosphokinase (IPK) family. This protein is likely responsible for the conversion of inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5). It may also convert 1,3,4,5,6-pentakisphosphate (InsP5) to PP-InsP4. Alternative splicing occurs for this gene, however, the full-length nature of all transcript variants has not yet been described.

Cellular Localization: Cytoplasm , Nucleus

Molecular Weight: 50 kDa

Gene ID: 9807

UniProt: Q92551