Pyruvate Dehydrogenase E1 alpha (Center) antibody

Catalog No. ABIN2855747

There is 1 publication for this product available. The Rabbit Polyclonal anti-Pyruvate Dehydrogenase E1 alpha antibody is suitable to detect Pyruvate Dehydrogenase E1 alpha in samples from Human, Mouse and Rat. It has been validated for WB, IF, IP, ICC and IHC (p).

Quick Overview for Pyruvate Dehydrogenase E1 alpha (Center) antibody (ABIN2855747)

Target: Pyruvate Dehydrogenase E1 alpha

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: Center

Cross-Reactivity: Rhesus Monkey, Chimpanzee

Cross-Reactivity (Details): Rhesus Monkey (100 %), Chimpanzee (100 %)

Characteristics: Rabbit Polyclonal antibody to Pyruvate Dehydrogenase E1 alpha (pyruvate dehydrogenase (lipoamide) alpha 1)
Pyruvate Dehydrogenase E1 alpha antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Pyruvate Dehydrogenase E1 alpha. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:1000-1:10000*

Comment:

Positive Control: HepG2 , Molt-4 , mouse brain , rat brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References

Chao, Wroblewski, Ilkayeva, Stevens, Bain, Meyer, Schenk, Martinez, Vergnes, Narkar, Drew, Hong, Boyadjian, Hevener, Evans, Reue, Spencer, Newgard, Tontonoz: "Skeletal muscle Nur77 expression enhances oxidative metabolism and substrate utilization." in: Journal of lipid research, Vol. 53, Issue 12, pp. 2610-9, (2012) (PubMed).

Target Details

Target: Pyruvate Dehydrogenase E1 alpha

Background: The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1), dihydrolipoyl transacetylase (DLAT, MIM 608770) (E2, EC 2.3.1.12), and dihydrolipoyl dehydrogenase (DLD, MIM 238331) (E3, EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM]

Cellular Localization: Mitochondrion matrix

Molecular Weight: 43 kDa

Gene ID: 5160