PON2 antibody

Catalog No. ABIN2855759

The Rabbit Polyclonal anti-PON2 antibody has been validated for WB and IHC (p). It is suitable to detect PON2 in samples from Human. There is 1 publication available.

Quick Overview for PON2 antibody (ABIN2855759)

Target: PON2 (Paraoxonase 2 (PON2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PON2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PON2 Antibody

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to PON2 (paraoxonase 2)
PON2 antibody [N1C2]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PON2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431 , Mouse liver

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-PON2 antibody (ABIN2855759)

Devarajan, Su, Grijalva, Yalamanchi, Yalamanchi, Gao, Trost, Nwokedi, Farias-Eisner, Farias-Eisner, Fogelman, Reddy: "Paraoxonase 2 overexpression inhibits tumor development in a mouse model of ovarian cancer." in: Cell death & disease, Vol. 9, Issue 3, pp. 392, (2019) (PubMed).

Target Details for PON2

Target: PON2 (Paraoxonase 2 (PON2))

Alternative Name: paraoxonase 2

Background: This gene encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described.

Cellular Localization: Membrane, Peripheral membrane protein

Molecular Weight: 39 kDa

Gene ID: 5445

UniProt: Q15165