NDST3 antibody (Center)

Catalog No. ABIN2855851

This Rabbit Polyclonal antibody specifically detects NDST3 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for NDST3 antibody (Center) (ABIN2855851)

Target: NDST3 (N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 3 (NDST3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDST3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NDST3 Antibody

Binding Specificity: Center

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Cow (Bovine)

Cross-Reactivity (Details): Mouse (92 %), Rat (91 %), Bovine (93 %)

Characteristics: Rabbit Polyclonal antibody to NDST3 (N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3)
NDST3 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human NDST3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: U87-MG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NDST3

Target: NDST3 (N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 3 (NDST3))

Alternative Name: NDST3

Background: This gene encodes a member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family. The encoded enzyme is a type II transmembrane protein that resides in the Golgi apparatus. This monomeric bifunctional enzyme catalyzes the N-deacetylation and N-sulfation of N-acetylglucosamine residues in heparan sulfate and heparin, which are the initial chemical modifications required for the biosynthesis of the functional oligosaccharide sequences that define the specific ligand binding activities of heparan sulfate and heparin.

Cellular Localization: Golgi apparatus membrane, Single-pass type II membrane protein

Molecular Weight: 101 kDa

Gene ID: 9348

Pathways: Glycosaminoglycan Metabolic Process