ATP5G1 antibody (Center)

Catalog No. ABIN2855894

There is 1 publication for this product available. The Rabbit Polyclonal anti-ATP5G1 antibody is suitable to detect ATP5G1 in samples from Human. It has been validated for WB.

Quick Overview for ATP5G1 antibody (Center) (ABIN2855894)

Target: ATP5G1 (ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit C1 (Subunit 9) (ATP5G1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP5G1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATP5G1 Antibody

Binding Specificity: Center

Cross-Reactivity: Mouse (Murine), Sheep (Ovine), Rhesus Monkey, Cow (Bovine)

Cross-Reactivity (Details): Mouse (80 %), Sheep (86 %), Rhesus Monkey (93 %), Bovine (85 %)

Characteristics: Rabbit polyclonal antibody to ATP synthase C1(mitochondrial) (ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C1 (subunit 9))
ATP5G1 antibody [N1C1]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ATP5G1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceWestern blot1:500-1:3000*

Comment:

Positive Control: Molt-4 , Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 40 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-ATP5G1 antibody (ABIN2855894)

Muluhngwi, Alizadeh-Rad, Vittitow, Kalbfleisch, Klinge: "The miR-29 transcriptome in endocrine-sensitive and resistant breast cancer cells." in: Scientific reports, Vol. 7, Issue 1, pp. 5205, (2019) (PubMed).

Target Details for ATP5G1

Target: ATP5G1 (ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit C1 (Subunit 9) (ATP5G1))

Alternative Name: ATP5G1

Background: This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene is one of three genes that encode subunit c of the proton channel. Each of the three genes have distinct mitochondrial import sequences but encode the identical mature protein. Alternatively spliced transcript variants encoding the same protein have been identified.

Cellular Localization: Mitochondrion membrane

Molecular Weight: 14 kDa

Gene ID: 516

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process