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MAD2L1 antibody (C-Term)

The Rabbit Polyclonal anti-MAD2L1 antibody has been validated for WB, IF, IP, IHC (p) and ICC. It is suitable to detect MAD2L1 in samples from Human and Rat.
Catalog No. ABIN2855897

Quick Overview for MAD2L1 antibody (C-Term) (ABIN2855897)

Target

See all MAD2L1 Antibodies
MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))

Reactivity

  • 69
  • 11
  • 8
  • 6
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
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Human, Rat

Host

  • 59
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  • 1
Rabbit

Clonality

  • 52
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Polyclonal

Conjugate

  • 39
  • 3
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  • 2
  • 2
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  • 2
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  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This MAD2L1 antibody is un-conjugated

Application

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  • 14
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  • 3
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  • 1
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Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
  • Binding Specificity

    • 15
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    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    C-Term

    Cross-Reactivity

    Cow (Bovine), Mouse (Murine), Rhesus Monkey

    Cross-Reactivity (Details)

    Mouse (92 %), Rhesus Monkey (100 %), Bovine (100 %)

    Characteristics

    Rabbit Polyclonal antibody to MAD2L1 (MAD2 mitotic arrest deficient-like 1 (yeast))
    MAD2L1 antibody [C2C3], C-term

    Purification

    Purified by antigen-affinity chromatography.

    Immunogen

    Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MAD2L1. The exact sequence is proprietary.

    Isotype

    IgG
  • Application Notes

    Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

    Comment

    Positive Control: NCIN87, U2OS, Hep G2, Molt4, Raji

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    1XPBS, 40 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

    Preservative

    Thimerosal (Merthiolate)

    Precaution of Use

    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Target

    MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))

    Alternative Name

    MAD2L1

    Background

    MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14.

    Molecular Weight

    24 kDa

    Gene ID

    4085
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