CYP7B1 antibody (C-Term)

Catalog No. ABIN2855947

This Rabbit Polyclonal antibody specifically detects CYP7B1 in WB, IHC (p), IF and ICC. It exhibits reactivity toward Human.

Quick Overview for CYP7B1 antibody (C-Term) (ABIN2855947)

Target: CYP7B1 (Cytochrome P450, Family 7, Subfamily B, Polypeptide 1 (CYP7B1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP7B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-CYP7B1 Antibody

Binding Specificity: C-Term

Characteristics: Rabbit polyclonal antibody to CYP7B1 (cytochrome P450, family 7, subfamily B, polypeptide 1)
CYP7B1 antibody [C3], C-term

Purification: Affinity purified by Protein A.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CYP7B1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , H1299

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2 mg/mL

Buffer: 1XPBS, 40 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for CYP7B1

Target: CYP7B1 (Cytochrome P450, Family 7, Subfamily B, Polypeptide 1 (CYP7B1))

Alternative Name: CYP7B1

Background: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis.

Cellular Localization: Endoplasmic reticulum membrane , Microsome membrane

Molecular Weight: 58 kDa

Gene ID: 9420

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Biosynthesis, Regulation of Intracellular Steroid Hormone Receptor Signaling