HADH antibody

Catalog No. ABIN2856051

The Rabbit Polyclonal anti-HADH antibody (ABIN2856051) specifically detects HADH in WB, IP, IHC (p), ICC and IF. The antibody is reactive with Human samples.

Quick Overview for HADH antibody (ABIN2856051)

Target: HADH (Hydroxyacyl-CoA Dehydrogenase (HADH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HADH antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (IF)

Grade: KO Validated

Product Details anti-HADH Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to HADH (hydroxyacyl-CoA dehydrogenase)
HADH antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human HADH. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: GL261 , C8D30 , PC-12 , 293T , A431 , HeLa , HepG2

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for HADH

Target: HADH (Hydroxyacyl-CoA Dehydrogenase (HADH))

Alternative Name: hydroxyacyl-CoA dehydrogenase

Background: This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene.

Cellular Localization: Mitochondrion matrix

Molecular Weight: 34 kDa

Gene ID: 3033

UniProt: Q16836

Pathways: Negative Regulation of Hormone Secretion, Monocarboxylic Acid Catabolic Process