PCCB antibody (Center)

Catalog No. ABIN2856061

This Rabbit Polyclonal antibody specifically detects PCCB in WB, IF, ICC and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for PCCB antibody (Center) (ABIN2856061)

Target: PCCB (Propionyl CoA Carboxylase beta Polypeptide (PCCB))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCCB antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PCCB Antibody

Binding Specificity: Center

Cross-Reactivity: Cow (Bovine), Pig (Porcine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)

Cross-Reactivity (Details): Pig (94 %), Rat (91 %), Xenopus laevis (90 %), Zebrafish (90 %), Bovine (97 %)

Characteristics: Rabbit Polyclonal antibody to PCCB (propionyl Coenzyme A carboxylase, beta polypeptide)
PCCB antibody [N2C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PCCB. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.54 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PCCB

Target: PCCB (Propionyl CoA Carboxylase beta Polypeptide (PCCB))

Alternative Name: PCCB

Background: The protein encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2).

Cellular Localization: Mitochondrion matrix

Molecular Weight: 58 kDa

Gene ID: 5096

Pathways: Monocarboxylic Acid Catabolic Process