NK2 Homeobox 5 antibody

Catalog No. ABIN2856166

This Rabbit Polyclonal antibody specifically detects NK2 Homeobox 5 in WB, IF, ICC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for NK2 Homeobox 5 antibody (ABIN2856166)

Target: NK2 Homeobox 5 (NKX2-5)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NK2 Homeobox 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NK2 Homeobox 5 Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to Nkx2.5 (NK2 transcription factor related, locus 5 (Drosophila))
Nkx2.5 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Nkx2.5. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Validation: Comparison

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.21 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7), 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NK2 Homeobox 5

Target: NK2 Homeobox 5 (NKX2-5)

Alternative Name: NK2 homeobox 5

Background: Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development (Shiojima et al., 1995 [PubMed 7665173]). It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.[supplied by OMIM]

Cellular Localization: Nucleus

Molecular Weight: 35 kDa

Gene ID: 1482

UniProt: P52952

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development