SEC31A antibody (N-Term)

Catalog No. ABIN2856188

The Rabbit Polyclonal anti-SEC31A antibody has been validated for WB and IHC (p). It is suitable to detect SEC31A in samples from Human.

Quick Overview for SEC31A antibody (N-Term) (ABIN2856188)

Target: SEC31A (SEC31 Homolog A (SEC31A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SEC31A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SEC31A Antibody

Binding Specificity: N-Term

Cross-Reactivity: Mouse (Murine), Chicken, Rat (Rattus), Zebrafish (Danio rerio), Cow (Bovine)

Cross-Reactivity (Details): Mouse (98 %), Chicken (93 %), Rat (97 %), Zebrafish (81 %), Bovine (97 %)

Characteristics: Rabbit Polyclonal antibody to SEC31A (SEC31 homolog A (S. cerevisiae))
SEC31A antibody [N1N3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human SEC31A. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: A549 , HeLa , Jurkat , NT2D1

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.7 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for SEC31A

Target: SEC31A (SEC31 Homolog A (SEC31A))

Alternative Name: SEC31A

Background: The protein encoded by this gene is similar to yeast Sec31 protein. Yeast Sec31 protein is known to be a component of the COPII protein complex which is responsible for vesicle budding from endoplasmic reticulum (ER). This protein was found to colocalize with SEC13, one of the other components of COPII , in the subcellular structures corresponding to the vesicle transport function. An immunodepletion experiment confirmed that this protein is required for ER-Golgi transport. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 133 kDa

Gene ID: 22872

Pathways: ER-Nucleus Signaling