STAM antibody (Center)

Catalog No. ABIN2856220

This Rabbit Polyclonal antibody specifically detects STAM in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for STAM antibody (Center) (ABIN2856220)

Target: STAM (Signal Transducing Adaptor Molecule (SH3 Domain and ITAM Motif) 1 (STAM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-STAM Antibody

Binding Specificity: Center

Cross-Reactivity: Mouse (Murine), Xenopus laevis, Cow (Bovine), Xenopus tropicalis

Cross-Reactivity (Details): Mouse (96 %), Xenopus laevis (88 %), Bovine (97 %), Xenopus tropicalis (90 %)

Characteristics: Rabbit Polyclonal antibody to STAM (signal transducing adaptor molecule (SH3 domain and ITAM motif) 1)
STAM antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human STAM. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for STAM

Target: STAM (Signal Transducing Adaptor Molecule (SH3 Domain and ITAM Motif) 1 (STAM))

Alternative Name: STAM

Background: This gene was identified by the rapid tyrosine-phosphorylation of its product in response to cytokine stimulation. The encoded protein contains a SH3 domain and the immunoreceptor tyrosine-based activation motif (ITAM). This protein associates with JAK3 and JAK2 kinases via its ITAM region, and is phosphorylated by the JAK kinases upon cytokine stimulation, which suggests the function of this protein is as an adaptor molecule involved in the downstream signaling of cytokine receptors. HGS/HRS (hepatocyte grwoth factor-regulated tyrosine kinase substrate) has been found to bind and counteract the function of this protein.

Cellular Localization: Cytoplasm (Probable) , Early endosome membrane, Peripheral membrane protein, Cytoplasmic side

Molecular Weight: 59 kDa

Gene ID: 8027

Pathways: EGFR Signaling Pathway, EGFR Downregulation