PIGK antibody

Catalog No. ABIN2856230

The Rabbit Polyclonal anti-PIGK antibody has been validated for WB and IHC (p). It is suitable to detect PIGK in samples from Human.

Quick Overview for PIGK antibody (ABIN2856230)

Target: PIGK (GPI8) (GPI-anchor transamidase (GPI8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIGK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PIGK Antibody

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to PIGK (phosphatidylinositol glycan anchor biosynthesis, class K)
PIGK antibody [N1C2]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PIGK. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , 293T

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.74 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PIGK

Target: PIGK (GPI8) (GPI-anchor transamidase (GPI8))

Alternative Name: phosphatidylinositol glycan anchor biosynthesis class K

Background: This gene encodes a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins.

Cellular Localization: Endoplasmic reticulum membrane, Single-pass type I membrane protein

Molecular Weight: 45 kDa

Gene ID: 10026

UniProt: Q92643