WNT11 antibody (Center)

Catalog No. ABIN2856232

This Rabbit Polyclonal antibody specifically detects WNT11 in WB, IHC, ICC, IHC (p) and IF. It exhibits reactivity toward Human and Mouse. It has been mentioned in 2+ publications

Quick Overview for WNT11 antibody (Center) (ABIN2856232)

Target: WNT11 (Wingless-Type MMTV Integration Site Family, Member 11 (WNT11))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WNT11 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-WNT11 Antibody

Binding Specificity: Center

Cross-Reactivity: Chicken, Rat (Rattus), Xenopus laevis, Cow (Bovine)

Cross-Reactivity (Details): Chicken (85 %), Rat (96 %), Xenopus laevis (81 %), Bovine (98 %)

Characteristics: Rabbit Polyclonal antibody to WNT11 (wingless-type MMTV integration site family, member 11)
WNT11 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human WNT11. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* Immunohistochemistry Assay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* ImmunohistochemistryAssay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , HeLa , HepG2 , mouse heart

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-WNT11 antibody (ABIN2856232)

Glaeser, Urban, Fenech, Voloshanenko, Kranz, Lari, Christianson, Boutros: "ERAD-dependent control of the Wnt secretory factor Evi." in: The EMBO journal, Vol. 37, Issue 4, (2018) (PubMed).

Jain, Noordam, Hoshi, Vallania, Conrad: "Validating single-cell genomics for the study of renal development." in: Kidney international, Vol. 86, Issue 5, pp. 1049-55, (2015) (PubMed).

Target Details for WNT11

Target: WNT11 (Wingless-Type MMTV Integration Site Family, Member 11 (WNT11))

Alternative Name: WNT11

Background: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 97 % , 85 %, and 63 % amino acid identity with mouse, chicken, and Xenopus Wnt11 protein, respectively. This gene may play roles in the development of skeleton, kidney and lung, and is considered to be a plausible candidate gene for High Bone Mass Syndrome.

Cellular Localization: Secreted , extracellular space , extracellular matrix

Molecular Weight: 39 kDa

Gene ID: 7481

Pathways: WNT Signaling, Cell-Cell Junction Organization