CD2AP antibody

Catalog No. ABIN2856284

The Rabbit Polyclonal anti-CD2AP antibody has been validated for WB, ICC, IF and IHC (p). It is suitable to detect CD2AP in samples from Human.

Quick Overview for CD2AP antibody (ABIN2856284)

Target: CD2AP (Cd2ap) (CD2-Associated Protein (Cd2ap))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD2AP antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CD2AP Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to CD2AP (CD2-associated protein)
CD2AP antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human CD2AP. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A375 , Rat2 , A431 , HeLa , HepG2 , K562 , THP-1 , HL-60

Validation: Comparison, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.17 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for CD2AP

Target: CD2AP (Cd2ap) (CD2-Associated Protein (Cd2ap))

Alternative Name: CD2 associated protein

Background: This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease.

Cellular Localization: Cytoplasm , cytoskeleton , Cell projection , ruffle

Molecular Weight: 71 kDa

Gene ID: 23607

UniProt: Q9Y5K6