Flightless I antibody (Internal Region)

Catalog No. ABIN2856293

The Rabbit Polyclonal anti-Flightless I antibody has been validated for WB, IF and ICC. It is suitable to detect Flightless I in samples from Human and Mouse.

Quick Overview for Flightless I antibody (Internal Region) (ABIN2856293)

Target: Flightless I

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Flightless I antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Flightless I Antibody

Binding Specificity: Internal Region

Cross-Reactivity: Chicken, Rat (Rattus), Xenopus tropicalis

Cross-Reactivity (Details): Chicken (87 %), Rat (96 %), Xenopus tropicalis (81 %)

Characteristics: Rabbit Polyclonal antibody to Flightless I (flightless I homolog (Drosophila))
Flightless I antibody [N3C1], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Flightless I. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , H1299 , HeLaS3 , Molt-4 , Raji , mouse brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.88 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Flightless I

Target: Flightless I

Background: This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17.

Cellular Localization: Nucleus (By similarity) , Cytoplasm , cytoskeleton (By similarity) , Centrosome (By similarity)

Molecular Weight: 145 kDa

Gene ID: 2314