DBNL antibody (N-Term)

Catalog No. ABIN2856301

This Rabbit Polyclonal antibody specifically detects DBNL in WB, IF, IHC (p) and ICC. It exhibits reactivity toward Human.

Quick Overview for DBNL antibody (N-Term) (ABIN2856301)

Target: DBNL (Drebrin-Like (DBNL))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DBNL antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-DBNL Antibody

Binding Specificity: N-Term

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Cow (Bovine)

Cross-Reactivity (Details): Mouse (92 %), Rat (92 %), Bovine (84 %)

Characteristics: Rabbit Polyclonal antibody to HIP55 (drebrin-like)
HIP55 antibody [N1], N-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HIP55. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: HeLaS3 , Molt-4

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for DBNL

Target: DBNL (Drebrin-Like (DBNL))

Alternative Name: HIP55

Background: Actin-binding adapter protein. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Its association with dynamin suggests that it may also connect the actin cytoskeleton to endocytic function. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. Binds to F-actin but is not involved in actin polymerization, capping or bundling. Does not bind G-actin.

Cellular Localization: Cytoplasm , cytoskeleton , Cell projection , lamellipodium

Molecular Weight: 48 kDa

Gene ID: 28988

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization