PPP2CA antibody (C-Term)

Catalog No. ABIN2856333

This anti-PPP2CA antibody is a Rabbit Polyclonal antibody detecting PPP2CA in WB, IF, IHC (p), IP and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for PPP2CA antibody (C-Term) (ABIN2856333)

Target: PPP2CA (Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP2CA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-PPP2CA Antibody

Binding Specificity: C-Term

Cross-Reactivity: Rat (Rattus), Zebrafish (Danio rerio), Xenopus laevis, Xenopus tropicalis, Dog (Canine), Pig (Porcine), Rabbit, Chicken, Cow (Bovine)

Cross-Reactivity (Details): Rat (100 %), Zebrafish (100 %), Xenopus laevis (100 %), Xenopus Tropicalis (100 %), Dog (100 %), Pig (100 %), Rabbit (100 %), Chicken (100 %), Bovine (100 %)

Characteristics: Rabbit Polyclonal antibody to PP2A alpha (protein phosphatase 2, catalytic subunit, alpha isozyme)
PP2A alpha antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PP2A alpha. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:1000-1:10000*

Comment:

Positive Control: 293T , A431 , H1299 , HeLaS3 , Molt-4 , Raji , mouse brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.479 mg/mL

Buffer: 1XPBS, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PPP2CA

Target: PPP2CA (Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA))

Alternative Name: PP2A alpha

Background: This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.

Cellular Localization: Cytoplasm , Nucleus , Centromere , Spindle

Molecular Weight: 36 kDa

Gene ID: 5515

Pathways: PI3K-Akt Signaling, Mitotic G1-G1/S Phases, Hepatitis C, Toll-Like Receptors Cascades