BTAF1 antibody (C-Term)

Catalog No. ABIN2856344

The Rabbit Polyclonal anti-BTAF1 antibody has been validated for IP and WB. It is suitable to detect BTAF1 in samples from Human.

Quick Overview for BTAF1 antibody (C-Term) (ABIN2856344)

Target: BTAF1 (TBP-Associated Factor 172 (BTAF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BTAF1 antibody is un-conjugated

Application: Immunoprecipitation (IP), Western Blotting (WB)

Product Details anti-BTAF1 Antibody

Binding Specificity: C-Term

Characteristics: Rabbit Polyclonal antibody to TAF172 (BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170 kDa (Mot1 homolog, S. cerevisiae))
TAF172 antibody [C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TAF172. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceImmunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for BTAF1

Target: BTAF1 (TBP-Associated Factor 172 (BTAF1))

Alternative Name: TAF172

Background: Initiation of transcription by RNA polymerase II requires the assistance of TATA box-binding protein (TBP, MIM 600075) and TBP-associated factors, or TAFs (e.g., TAF2B, MIM 604912), in 2 distinct complexes, TFIID and B-TFIID. The TFIID complex is composed of TBP and more than 8 TAFs. However, the majority of TBP is present in the B-TFIID complex, which is composed of TBP and TAFII170, also called TAF172, and has DNA-dependent ATPase activity.[supplied by OMIM]

Cellular Localization: Nucleus

Molecular Weight: 207 kDa

Gene ID: 9044