NUP50 antibody (C-Term)

Catalog No. ABIN2856374

The Rabbit Polyclonal anti-NUP50 antibody has been validated for WB, IF, ICC and IHC (p). It is suitable to detect NUP50 in samples from Human.

Quick Overview for NUP50 antibody (C-Term) (ABIN2856374)

Target: NUP50 (Nucleoporin 50kDa (NUP50))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUP50 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NUP50 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to NUP50 (nucleoporin 50 kDa)
NUP50 antibody [C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NUP50. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: mouse testis

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.79 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NUP50

Target: NUP50 (Nucleoporin 50kDa (NUP50))

Alternative Name: nucleoporin 50

Background: The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Nucleus , nuclear pore complex , Nucleus membrane, Peripheral membrane protein, Nucleoplasmic side (By similarity)

Molecular Weight: 50 kDa

Gene ID: 10762

UniProt: Q9UKX7

Pathways: Tube Formation