Midline 2 antibody (C-Term)

Catalog No. ABIN2856379

This Rabbit Polyclonal antibody specifically detects Midline 2 in WB. It exhibits reactivity toward Human.

Quick Overview for Midline 2 antibody (C-Term) (ABIN2856379)

Target: Midline 2 (MID2)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Midline 2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Midline 2 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to MID2 (midline 2)
MID2 antibody [C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MID2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: MID2 transfected 293T

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Midline 2

Target: Midline 2 (MID2)

Alternative Name: midline 2

Background: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms.

Cellular Localization: Cytoplasm , cytoskeleton

Molecular Weight: 83 kDa

Gene ID: 11043

UniProt: Q9UJV3