PEF1 antibody

Catalog No. ABIN2856405

This anti-PEF1 antibody is a Rabbit Polyclonal antibody detecting PEF1 in WB, IHC (p), ICC and IF. Suitable for Human.

Quick Overview for PEF1 antibody (ABIN2856405)

Target: PEF1 (Penta-EF-Hand Domain Containing 1 (PEF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PEF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-PEF1 Antibody

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to PEF1 (penta-EF-hand domain containing 1)
PEF1 antibody [N2C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PEF1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PEF1

Target: PEF1 (Penta-EF-Hand Domain Containing 1 (PEF1))

Alternative Name: penta-EF-hand domain containing 1

Background: PEF1 is a Ca(2+)-binding protein that belongs to the penta-EF-hand (PEF) protein family, which includes the calpain small subunit (CAPNS1, MIM 114170), sorcin (SRI, MIM 182520), grancalcin (GCA, MIM 607030), and ALG2 (PDCD6, MIM 601057) (Kitaura et al., 2001 [PubMed 11278427]).[supplied by OMIM]

Cellular Localization: Cytoplasm , Membrane, Peripheral membrane protein

Molecular Weight: 30 kDa

Gene ID: 553115

UniProt: Q9UBV8