NAPA antibody (Center)

Catalog No. ABIN2856408

This anti-NAPA antibody is a Rabbit Polyclonal antibody detecting NAPA in WB, IP, ICC, IF and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for NAPA antibody (Center) (ABIN2856408)

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAPA antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NAPA Antibody

Binding Specificity: Center

Cross-Reactivity: Rhesus Monkey, Chimpanzee

Cross-Reactivity (Details): Rhesus Monkey (100 %), Chimpanzee (100 %)

Characteristics: Rabbit Polyclonal antibody to alpha SNAP (N-ethylmaleimide-sensitive factor attachment protein, alpha)
alpha SNAP antibody [N2C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha SNAP. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation Assay-dependent dilution Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* ImmunoprecipitationAssay-dependent dilution Western blot1:1000-1:10000*

Comment:

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NAPA

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Alternative Name: alpha SNAP

Background: The 'SNARE hypothesis' is a model explaining the process of docking and fusion of vesicles to their target membranes. According to this model, membrane proteins from the vesicle (v-SNAREs) and proteins from the target membrane (t-SNAREs) govern the specificity of vesicle targeting and docking through mutual recognition. Once the 2 classes of SNAREs bind to each other, they form a complex that recruits the general elements of the fusion apparatus, namely NSF (N-ethylmaleimide-sensitive factor) and SNAPs (soluble NSF-attachment proteins), to the site of membrane fusion, thereby forming the 20S fusion complex. Alpha- and gamma-SNAP are found in a wide range of tissues and act synergistically in intra-Golgi transport. The sequence of the predicted 295-amino acid human protein encoded by NAPA shares 37 % , 60 %, and 67 % identity with the sequences of yeast, Drosophila, and squid alpha-SNAP, respectively. Platelets contain some of the same proteins, including NSF, p115/TAP, alpha-SNAP, gamma-SNAP, and the t-SNAREs syntaxin-2 and syntaxin-4, that are used in many vesicular transport processes in other cell types. Platelet exocytosis uses a molecular mechanism similar to that used by other secretory cells, such as neurons, although the proteins used by the platelet and their modes of regulation may be quite different.

Cellular Localization: Membrane, Peripheral membrane protein

Molecular Weight: 33 kDa

Gene ID: 8775

Pathways: Synaptic Vesicle Exocytosis, Asymmetric Protein Localization