NAPA antibody

Catalog No. ABIN306712

This Mouse Monoclonal antibody specifically detects NAPA in WB, IP and EIA. It exhibits reactivity toward Human, Rat and Cow.

Quick Overview for NAPA antibody (ABIN306712)

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Reactivity: Human, Rat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This NAPA antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: 15D4

Product Details anti-NAPA Antibody

Purpose: Mouse anti Human alpha SNAP

Specificity: Human alpha -SNAP

Cross-Reactivity: Cow, Human, Rat

Characteristics: SNAPs (soluble NSF attachment proteins), acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicle-to-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNARE. Beta-SNAP (or SNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20 S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process. Whereas alpha-SNAP is expressed in all mammalian tissues, beta-SNAP is expressed only in brain.

Purification: The antibody was purified to >95% homogeneity by standard chromatographic techniques

Immunogen: Balb/C mice were immunized with recombinant human alpha -SNAP. The resultant hybridomas were selected for reactivity with the immunogen by ELISA and with the antigen by Western blot.

Isotype: IgG1

Application Details

Application Notes: Optimal concentration should be evaluated by serial dilutions. Clone 4E4 anti-?SNAP specifically recognizes ?-SNAP as a single band of ~36 kDa on western blot of rat kidney, rat brain and MDBK cells and does not recognize ?-SNAP Application: A dilution of 0.5-5 μg/mL is suggested for western blot and 2-10 μg/mL for immunoprecipitation.

Restrictions: For Research Use only

Handling

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for NAPA

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Alternative Name: alpha SNAP

Background: Alpha/beta SNAP, mouse anti alpha soluble NSF attachment protein,SNAPA

UniProt: P54920

Pathways: Synaptic Vesicle Exocytosis, Asymmetric Protein Localization