CHMP5 antibody

Catalog No. ABIN2856420

The Rabbit Polyclonal anti-CHMP5 antibody (ABIN2856420) specifically detects CHMP5 in WB, IF, IHC (p), ICC and IP. The antibody is reactive with Human samples.

Quick Overview for CHMP5 antibody (ABIN2856420)

Target: CHMP5 (Charged Multivesicular Body Protein 5 (CHMP5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHMP5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-CHMP5 Antibody

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to CHMP5 (chromatin modifying protein 5)
CHMP5 antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human CHMP5. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2

Validation: Comparison, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.7 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for CHMP5

Target: CHMP5 (Charged Multivesicular Body Protein 5 (CHMP5))

Alternative Name: charged multivesicular body protein 5

Background: CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM]

Cellular Localization: Cytoplasm , cytosol , Endosome membrane, Peripheral membrane protein , Cytoplasm , cytosol , Endosome membrane

Molecular Weight: 25 kDa

Gene ID: 51510

UniProt: Q9NZZ3