NSMCE1 antibody (Center)

Catalog No. ABIN2856490

This Rabbit Polyclonal antibody specifically detects NSMCE1 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for NSMCE1 antibody (Center) (ABIN2856490)

Target: NSMCE1 (Non-SMC Element 1 Homolog (NSMCE1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSMCE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NSMCE1 Antibody

Binding Specificity: Center

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Cow (Bovine)

Cross-Reactivity (Details): Mouse (90 %), Rat (92 %), Bovine (90 %)

Characteristics: Rabbit polyclonal antibody to NSMCE1 (non-SMC element 1 homolog (S. cerevisiae))
NSMCE1 antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human NSMCE1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NSMCE1

Target: NSMCE1 (Non-SMC Element 1 Homolog (NSMCE1))

Alternative Name: NSMCE1

Background: Probable component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks.

Cellular Localization: Nucleus

Molecular Weight: 31 kDa

Gene ID: 197370

Pathways: Positive Regulation of Response to DNA Damage Stimulus