RIC8A antibody (C-Term)

Catalog No. ABIN2856525

This anti-RIC8A antibody is a Rabbit Polyclonal antibody detecting RIC8A in WB and IHC (p). Suitable for Human.

Quick Overview for RIC8A antibody (C-Term) (ABIN2856525)

Target: RIC8A (Resistance To Inhibitors of Cholinesterase 8 Homolog A (C. Elegans) (RIC8A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIC8A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RIC8A Antibody

Binding Specificity: C-Term

Cross-Reactivity: Rat (Rattus), Cow (Bovine)

Cross-Reactivity (Details): Rat (100 %), Bovine (100 %)

Characteristics: Rabbit Polyclonal antibody to RIC8A (resistance to inhibitors of cholinesterase 8 homolog A (C. elegans))
RIC8A antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RIC8A. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: A431 , H1299 , HeLa , HepG2 , Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.85 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for RIC8A

Target: RIC8A (Resistance To Inhibitors of Cholinesterase 8 Homolog A (C. Elegans) (RIC8A))

Alternative Name: RIC8A

Background: Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.

Molecular Weight: 60 kDa

Gene ID: 60626

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, Asymmetric Protein Localization