Nemo-Like Kinase antibody

Catalog No. ABIN2856544

This Rabbit Polyclonal antibody specifically detects Nemo-Like Kinase in WB, IF, IHC (p) and ICC. It exhibits reactivity toward Human.

Quick Overview for Nemo-Like Kinase antibody (ABIN2856544)

Target: Nemo-Like Kinase (NLK)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nemo-Like Kinase antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-Nemo-Like Kinase Antibody

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to NLK (nemo-like kinase)
NLK antibody [N3C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human NLK. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , HeLa , NLK-transfected 293T

Validation: Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Nemo-Like Kinase

Target: Nemo-Like Kinase (NLK)

Alternative Name: nemo like kinase

Background: Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP (By similarity). Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3.

Cellular Localization: Nucleus (By similarity) , Cytoplasm (By similarity)

Molecular Weight: 58 kDa

Gene ID: 51701

UniProt: Q9UBE8

Pathways: Ubiquitin Proteasome Pathway