ITPK1 antibody

Catalog No. ABIN2856549

This anti-ITPK1 antibody is a Rabbit Polyclonal antibody detecting ITPK1 in WB, IF and ICC. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for ITPK1 antibody (ABIN2856549)

Target: ITPK1 (Inositol-Tetrakisphosphate 1-Kinase (ITPK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITPK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ITPK1 Antibody

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to ITPK1 (inositol 1,3,4-triphosphate 5/6 kinase)
ITPK1 antibody [N3C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ITPK1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.53 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-ITPK1 antibody (ABIN2856549)

Dovey, Diep, Clarke, Hale, McNamara, Guo, Brown, Cao, Grace, Gough, Bertin, Dixon, Fiedler, Mocarski, Kaiser, Moldoveanu, York, Carette: "MLKL Requires the Inositol Phosphate Code to Execute Necroptosis." in: Molecular cell, Vol. 70, Issue 5, pp. 936-948.e7, (2019) (PubMed).

Target Details for ITPK1

Target: ITPK1 (Inositol-Tetrakisphosphate 1-Kinase (ITPK1))

Alternative Name: inositol-tetrakisphosphate 1-kinase

Background: Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3. Phosphorylates Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5. This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca(2+)-activated Cl(-) channels, while Ins(1,3,4,5,6)P5 is not. Also phosphorylates Ins(1,3,4)P3 on O-5 and O-6 to form Ins(1,3,4,6)P4, an essential molecule in the hexakisphosphate (InsP6) pathway. Also acts as an inositol polyphosphate phosphatase that dephosphorylate Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 to Ins(1,3,4)P3, and Ins(1,3,4,5,6)P5 to Ins(3,4,5,6)P4. May also act as an isomerase that interconverts the inositol tetraphosphate isomers Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP and magnesium. Probably acts as the rate-limiting enzyme of the InsP6 pathway. Modifies TNF-alpha-induced apoptosis by interfering with the activation of TNFRSF1A-associated death domain.

Molecular Weight: 46 kDa

Gene ID: 3705

UniProt: Q13572